Only the very early version, “Steamboat Willie”. And only insofar as it is not a trademark. You can, however, perfectly legally use copyrighted characters. EG The copyrighted Mickey Mouse appears multiple times in South Park. Copyright ideologues always try to make people forget that. They want every human thought and every idea to be owned as “property” and used to extract rent money.
Only the very early version, “Steamboat Willie”. And only insofar as it is not a trademark. You can, however, perfectly legally use copyrighted characters. EG The copyrighted Mickey Mouse appears multiple times in South Park. Copyright ideologues always try to make people forget that. They want every human thought and every idea to be owned as “property” and used to extract rent money.